Journal: Cancers

Article Title: Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation

doi: 10.3390/cancers13081845

Figure Lengend Snippet: Flow cytometry antibodies used.

Article Snippet: CD36-PEVio770 , Miltenyi , 130-110-742 , REA770 , .

Techniques: Flow Cytometry, In Vivo, In Vitro

Journal: Cancers

Article Title: Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation

doi: 10.3390/cancers13081845

Figure Lengend Snippet: Murlentamab opsonization of SKOV3-R2 + orients naïve macrophages and reprograms TAMs towards an M1-like profile. SKOV3-R2 + ovarian tumor cells were labeled with different 3C23K antibodies (3C23K-FcKO control, 3C23K-CHO normally fucosylated or murlentamab the low fucosylated form) and cultured in the presence of human monocyte-derived macrophages from healthy donors unstimulated (M0) or stimulated with M-CSF and IL-10 (TAMs). ( A ) The proportion of macrophages expressing M1/M2 membrane markers (CD32, CD64, CD80, TLR2, CD163, CD36 and CD206) was determined by flow cytometry after three days of co-culture with SKOV3-R2 + cells. ( B ) The release of cytokines (IL1β, IL12, TNFα, IL6, IFNγ, IL10) and chemokines (CCL2, CCL4, CCL5, CXCL9 and CXCL10) in the culture medium was determined by AlphaLISA after three days of co-culture with SKOV3-R2 + cells. Data shown (boxplots) are the results from three different experiments (performed with three different healthy donors). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. p values were determined using one-way ANOVA analysis followed by Tukey’s multiple comparisons test.

Article Snippet: CD36-PEVio770 , Miltenyi , 130-110-742 , REA770 , .

Techniques: Labeling, Cell Culture, Derivative Assay, Expressing, Membrane, Flow Cytometry, Co-Culture Assay

Journal: Scientific Reports

Article Title: Endothelial Cell Phenotypes are Maintained During Angiogenesis in Cultured Microvascular Networks

doi: 10.1038/s41598-018-24081-z

Figure Lengend Snippet: Illustration of CD36 labeling in ex vivo cultured microvascular networks and stimulated in vivo microvasculature. Comparison between Day 3 ( Ex Vivo ) ( A–C ) and Day 3 ( In Vivo ) ( D–F ) microvascular networks revealed diminishing CD36 labeling along the length of the capillary sprout becoming absent at the tip. Arrows identify examples of CD36-negative capillary sprouts. Scale bars = 200 µm. ( G , H ) Capillary sprouts exhibited a substantial decrease in CD36 labeling compared to larger network vessels ( > 10 µm) in Day 3 ( Ex Vivo ) and Day 3 ( In Vivo ) tissues. White and black bars represent “Sprouts” and “Networks” respectively for Day 3 ( Ex Vivo ) and Day 3 ( In Vivo ) groups. The *** indicates a significant difference of p < 0.001 by Mann-Whitney U test.

Article Snippet: The following primary antibodies were used: UNC5b (1:100; Abcam; Cambridge, MA), VEGFR-2 (1:50; Santa Cruz Biotechnology; Dallas, TX), Alexa-568 Phalloidin (1:50; Invitrogen; Carlsbad, CA), CD36 (1:100; Novus Biologicals; Littleton, CO), and PECAM (1:200; BD Pharmingen; San Jose, CA).

Techniques: Labeling, Ex Vivo, Cell Culture, In Vivo, Comparison, MANN-WHITNEY

Journal: International Journal of Molecular Medicine

Article Title: Rosiglitazone attenuates atherosclerosis and increases high-density lipoprotein function in atherosclerotic rabbits

doi: 10.3892/ijmm.2015.2072

Figure Lengend Snippet: (A) ABCA1 protein expression in hepatocytes and peritoneal macrophages determined by flow cytometry. (B) SR-B1 protein expression in hepatocytes and peritoneal macrophages determined by flow cytometry. Data are presented as the means ± SEM, (n=6 in each group). # P<0.05, * P<0.01, ## P<0.001, vs. control group; ** P<0.01, *** P<0. 05, **** P<0. 001, vs. AS group (ANOVA). ABCA1, adenosine triphosphate binding cassette transporter A1; SR-B1, scavenger receptor class B type I; AS, atherosclerosis.

Article Snippet: Immunostaining for ABCA1 (Boster Biotechnology Co. Ltd., Wuhan, China) and SR-BI (Abcam Co. Ltd., Cambridge, MA, USA) was performed on paraffin-embedded aortic atherosclerotic sections using the specific antibody and a streptavidin-biotin peroxidase-complex (SABC).

Techniques: Expressing, Flow Cytometry, Control, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Rosiglitazone attenuates atherosclerosis and increases high-density lipoprotein function in atherosclerotic rabbits

doi: 10.3892/ijmm.2015.2072

Figure Lengend Snippet: (A) Hepatocyte ABCA1 mRNA expression quantified by RT-qPCR. (B) Hepatocyte SR-B1 mRNA expression quantified by RT-qPCR. Data are presented as the means ± SEM, (n=6 in each group). * P<0.01 vs. control group; ** P<0.01 vs. AS group (ANOVA). ABCA1, adenosine triphosphate binding cassette transporter A1; SR-B1, scavenger receptor class B type I; AS, atherosclerosis.

Article Snippet: Immunostaining for ABCA1 (Boster Biotechnology Co. Ltd., Wuhan, China) and SR-BI (Abcam Co. Ltd., Cambridge, MA, USA) was performed on paraffin-embedded aortic atherosclerotic sections using the specific antibody and a streptavidin-biotin peroxidase-complex (SABC).

Techniques: Expressing, Quantitative RT-PCR, Control, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Rosiglitazone attenuates atherosclerosis and increases high-density lipoprotein function in atherosclerotic rabbits

doi: 10.3892/ijmm.2015.2072

Figure Lengend Snippet: Aortic sections were subjected to immunohistochemical staining for ABCA1 protein localization. Representative images captured at magnification, ×40, ×100 and ×200. ABCA1, adenosine triphosphate binding cassette transporter A1; AS, atherosclerosis.

Article Snippet: Immunostaining for ABCA1 (Boster Biotechnology Co. Ltd., Wuhan, China) and SR-BI (Abcam Co. Ltd., Cambridge, MA, USA) was performed on paraffin-embedded aortic atherosclerotic sections using the specific antibody and a streptavidin-biotin peroxidase-complex (SABC).

Techniques: Immunohistochemical staining, Staining, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Rosiglitazone attenuates atherosclerosis and increases high-density lipoprotein function in atherosclerotic rabbits

doi: 10.3892/ijmm.2015.2072

Figure Lengend Snippet: Quantification of immunohistochemical staining for ABCA1 expression in aortic atherosclerotic lesions among the control, atherosclerotic and rosiglitazone groups at 12 weeks.

Article Snippet: Immunostaining for ABCA1 (Boster Biotechnology Co. Ltd., Wuhan, China) and SR-BI (Abcam Co. Ltd., Cambridge, MA, USA) was performed on paraffin-embedded aortic atherosclerotic sections using the specific antibody and a streptavidin-biotin peroxidase-complex (SABC).

Techniques: Immunohistochemical staining, Staining, Expressing, Control